Showing posts with label PH. Show all posts
Showing posts with label PH. Show all posts

Saturday, August 6, 2016

Of Plants, pH, CO2, Nitrates, and Water Quality

Starting up my new 29 Gallon tank has been the slowest setup I've ever done, in part because I became really focused on learning as much as I  could about water quality in the process. And plants definitely came before fish, to the ludicrous extreme that for too long already my plants have been starved of nitrogen: my nitrate readings have been zero for months now. I almost "had" to add more fish to introduce some pollution, because the fish wastes are food for the plants - so I'll be back to watching the water parameters like a hawk. This week I added three Otocinclus catfish and a school of a dozen Neon Tetras, which by accident included a single Lambchop Rasbora, which is a very interesting critter, with hilarious consequences...

About that lone Lambchop Rasbora

OK, so that lone Lambchop Rasbora was included by accident. What are you gonna do? Just watch what happens next...
Initially, it seemed as if he was just falling in line with the school of Neons. But then, after a few hours, a hilarious pattern emerged: the Rasbora falls in with the school for a while and then wanders off on his own - after all, he's a breed apart. So he explores the tank on his own, that is... until he sees a Neon that became separated from the school, and like a shepherd dog, he starts chasing him, and he continues to chase the Neons until the school is together again, and then for a while he swims with the school, until he is satisfied, and the pattern repeats itself. It will be very interesting to watch if this continues.

For now, I do not want to add more fish until I have a firm handle on the changes in water quality again, though considering my heavy plant growth, I think the new fish will barely move the needle on nitrates, but I also think it will take one or two months before I can tell for sure. I am continuing to take my time to find the right balance between fish and plants.

Hanna Instruments pH tester


Then it was time to turn to the mysteries of pH, that all important parameter, or so it seems. By the way, many smart pros in the field warn against an obsessive focus on pH, other parameters are at least equally important. As per usual, the some of the best source of information are the sections on water quality on the American Aquarium Products site:  Nitrogen Cycle, Redox Potential, and Carbonate Hardness (KH) - the point being that if you lose sight of the KH parameter, you are asking for pH instability.

Like most aquarists, I had forever been using the API water test kits for everything, including pH. Amazingly, I had been involved in some technology pertaining to drinking water, and had a set of electronic testers for that reason. Originally, I had kept them at my office, but when I brought them home, I forgot about them until recently, as I became deeply interested in aquarium water quality. My Hanna Instruments ORP and TDS meters were still in good working order, but my pH probe proved unreliable (they had all been purchased in 1999). Quite an amazing outcome, actually. I bought a new Hanna pH probe through American Aquarium Products (Hanna InstrumentsChecker (R) Plus pH Tester - HI98100). It has +/- 0.2 pH accuracy, and automatic calibration. After carefully calibrating it, I began to find that the API pH test (with the drops, I already did not like the strips...) is reasonably unreliable, but the greater accuracy of the HI98100 is worth it, because it provides a more precise and unambiguous reading. I am not saying the API test is unreliable, but it can be harder to read, however, you must be aware that the PH tester needs to be calibrated regularly, and for aquarium work the only calibration that matters is with the pH 7.0 reference liquid. You should do the calibration every time if you only use the pH probe irregularly, but then of course if you have multiple tanks that's still more convenient, and more accurate. If you test daily, Hanna recommends calibrating the tester once or twice a week. Sometimes I do have episode where I'm testing the water daily.

With three tanks, already the convenience of the electronic tester is a significant time saver, and the extra accuracy is a bonus, but I will be monitoring very carefully how all various measurements validate and reinforce my newly accurate pH readings, and to be sure I will periodically recalibrate my pH Checker, but I am sold on this product for any serious aquarist.   

Hydor CO2 kit

A good reference chart on KH and pH is the one on Think Fish, here. The bottom line is, for good CO2 levels in a typical freshwater tank, your KH should be between 3.5 and 10 dH, or between 50 and 200 ppm, which reinforces what I said above about not focusing on a single parameter. Now depending on the level of lighting, you may need some CO2 injection, for the plants otherwise may not get sufficient CO2.
In my quest for CO2 on a budget, I scooped up a Hydor CO2 Green NRG Advanced kit, which I would not recommend, because it is discontinued by the manufacturer. I only found that out after I bought it. If you can still find it, they're usually cheap, but you're buying a dead-end product, for which you can get no support. I should have checked the Hydor site first, and I would have realized that the product was orphaned, but... hindsight is always 20/20.

Having said that, for doing CO2 on a budget, the thing actually works sort of OK. I am quite a Hydor fan, so I can only hope some day they would come out with a new and improved version of this design. So far, the main problem I found with it is that it takes some effort to adjust it right, and the valve does tend to drift, so you need to check your bubbles once in a while. You can find a host of YouTube videos on this piece of equipment, such as this one, it's a nifty design, but don't mount it vertically like this video shows... it will work better horizontally, as shown here.

Besides the adjustment problem I've experienced, the fact is that it does not have a solenoid valve, which in and of itself would cost nearly as much as what I've paid for the whole system. I did put it on a timer however, so at night the CO2 (12 bubbles per minute), just bubbles up and escapes, whereas during the day the diffuser runs and that works really well, so the CO2 is really absorbed into the water very well. It is a nifty system that uses a little motor, similar to Hydor's Bubble Maker, for oxygenating your tank. Again, since it has been out of production for 8 years already, you cannot get any replacement parts for it any more, and it is only a matter of time until I will have to get a serious CO2 system. Economics also plays into it, for even on my 29 Gallon tank, I am now going through on 74g CO2 cartridge in 4-6 weeks, and they cost $10 each, where as a five pound CO2 tank would probably last a year or more on such a small tank, particularly with a solenoid valve, so it would be shut off at night, and a single charge costs me $20 around where I live.

CO2 wish list

I've been speccing CO2 systems for a while, and my guess is that my next move would be the Complete Ultimate CO2 System form Aquariumplants.com, here. That system truly looks awesome. The truth is a completely automated CO2 system is the way to go, especially for a larger tank with serious plants. Number one, by shutting it off at night, besides saving some CO2, you are reducing the risk of over supplying CO2, which is not good for the occupants. Your plants exhale CO2 at night, while they inhale CO2 by day. I used to have such a system when I ran a 125 Gallon tank for nearly 20 years, and it was quite the thing, to be able to simply regulate your pH automatically with such a system, rather than counting bubbles, as you have to do with any system that you need to regulate manually. (I do it once a week with my Hydor system, to make sure...)

Conclusion

Once you get serious about plants, you have the low-tech way, in which you balance fish and plant population, because fish waste is plant food. Diana Walstad is the big proponent of that model currently, but it is a very traditional approach, and all my earliest tanks in my childhood days were based on that principle. You should see this review by Dustin, and this interview with Diana Walstad by Aquarimax. 
If you are open to go hi-tech, with lots of lighting and CO2 injection, you may be able to get by with a manually regulated CO2 system in smaller tanks, and there are numerous options on the market. Generally speaking, the cheaper they are, the more work they require. Once you get into larger tanks, an automated CO2 system with a pH-probe that controls the CO2 diffusion is the way to go. At that level the idea that CO2 injection is extra work no longer applies. It will actually reduce the work, though you will have to trim your plants more often.


Wednesday, May 4, 2016

Duck weed or no duck weed

Traditionally, we were all taught to hate duck weed. It did not belong in the aquarium, supposedly. Recently Matt Owens' book, The Alternative Aquarium: A Robust Habitat changed my mind, and since then I've been studying a Dutch site on ecological aquarium keeping as well. And the more you start researching Duck weed online it gets positively interesting.

Duck weed hoovers up ammonia, nitrites and nitrates, and it oxygenates the water - and then to think people did not use to like this plant. There are many interesting posts and articles to be found:


It makes me wonder, how did I ever get by without it? The general principle seems to be that plants under water do not get but so much CO2, whereas plants that grow out of the water, or in this case, that float on top of the water, can absorb CO2 from the air, and therefore can grow much faster, and in the process absorb the fish wastes, which are nutrients to them.

Besides all of the wonders of sucking up nutrients at a fast rate, there is also the fact that it slows down evaporation, and therefore it is an alternative to a canopy, if you want to have an open tank...

As my new 29 gallon tank matured, and ammonia and nitrite finally went to zero, initially my nitrates were as high as 40 ppm, or even higher, upto 80 ppm at times, but duckweed was well... growing like a weed, and while I recently did a partial water change, I also just harvested some duckweed to see if it will indeed end up lowering the nitrate level, for my tank is fairly thinly populated, with just 5 amano shrimp (I think - though I started with 6...), and 5 pygmy cories. In the end however, with Duck weed (and Hornwort, and other plants), my nitrate reading is typically in the 5-10 PPM range, without water changes.

Here are some recent pictures:
4/28, with Duck Weed cover...

5/4 with the hidden LED to penetrate the cover
and some of the occupants:
5/4 pregnant Amano shrimp walking out of the picture
Ok, so I gave up trying to catch one of them in the picture, but there are three pregnant females... of the original three males I recently can find only two, so maybe one gave up the ghost during my two month tank cycling period, which seemed to go very slow at times.
Be that as it may, they do seem to be thriving, and my five Pygmy Cories, who don't pose well for pictures either, seem to be doing very well also.

The biggest challenge with the Duck Weed has been the lighting, for it not only slows evaporation, it also shields the light.

Duck weed and lighting

I was getting concerned with the weakness of the light, and it seemed evident to me that the plants needed more. 
The lighting has evolved to:
  • Hagen Flora Glo, which has the right color temperature for plant growth (2,700K). but low output (650 lumen/90 LUX). At some point, I may consider replacing this with a Life Glo Bulb, which has higher output (1,470 lumen, at 6,700K).
  • A Deep Blue Micro 3W LED SolarFlare, which gives the Wisteria some needed light. Color temperature is 6,700K daylight. 
  • A Marineland 21"Hidden LED that clamps to the tank frame inside the lid, and penetrates better through the duck weed, being right above the water line. It has 72 LEDs, with Par/Lux at 12" 25/1121 and at 24" 11/377, with 550 lumens, and the color temperature is 6,500K, which is daylight.
This is still not high intensity lighting you would need for serious plant growth, but with fairly regular, low light plants, it will definitely do. All in all it is a modest, but workable solution. The Wisteria is growing by leaps and bounds, and soon I'll be donating plants to the pet store... Two Amazon Swords produced three offshoots, which are catching on. The problem area is the excess shade below the massive Wisteria, so I will have to start pruning, which should help water quality again by stimulating growth... talking about which... the last few days I seem to have stabilized at pH 7.0 and ORP 85, with an rH in the 22-23 range, which should be excellent, except I still have no objective way to measure if there's actually any Hydrogen present in the water, though it might be the case by reason of the Wonder shells, especially because of the Magnesium.


Tuesday, March 29, 2016

Water Quality Revisited

It took me quite a while to start finding the good sources of information online, but it is finally coming together... American Aquarium Products is an absolutely amazing dealer, and their online tutorials are unsurpassed, if you want to run a thinking person's aquarium. The point being, keeping an aquarium is a learning exercise. You can sit and enjoy the beauty, but the excitement is about learning about the life that goes on inside. Beside the website for their business, with a proliferation of valuable technical information, AAP also maintains a separate informational site Everything Aquatic, and some online forums, the Everything Aquatic Forum. you can't beat this information source, and it has led me to research more deeply, among other things into water quality. I am quickly catching up after 20+ years without an aquarium in my life...

Eventually, I ended up connecting the dots regarding water quality, including my experience with water ionizers in the past, and my gradual discovery that so-called alkaline water is nonsense, except that yes, we need the electrolytes, but the alkalinity is not the first point, the low ORP (Oxidation Reduction Potential) is, preferably in the -150 to -200 range, but beyond that, I have now come to understand that the real measure you want to look at is the rH, the relative Hydrogen score, which I found documented on an amazing website by one Vinny Pinto, who has put the whole thing together.
The formula is:  rH = ((ORP+205)/29.58+(2xpH)).


29 gallon March 1st, 2016
29 Gallon on March 15th, Wisteria taking off.


Nitrogen cycle revisited

I did a more deliberate startup of my 29 gallon aquarium than ever before. Somebody gave it to me last September, and I have very slowly and deliberately planned the whole setup, and still now I wish I could do it all over again, and be even more methodical about it. For one thing, in the beginning I only did water tests once a week, and only later, when things where not going the way I thought they should go, did I start to test daily. If I had to do it over, I would suggest that for ANY aquarium startup the first thing to do is to get the Seachem Alert Combo. The Ammonia Alert lasts a full year, and even the pH alert is good for 3-6 months, in other words, this little device will see you through the whole startup period and well into 'normal' operations.

Everything I thought I knew about the Nitrogen cycle was useless, for recent research has shown that the nitrogen cycles is far more complex than previously thought, and all of this stuff has come out since I was away from aquariums for at least 20 years. Again, it was through reading on the AAP website, and their information about Aquarium Cycling and the Nitrogen cycle that I found my way to a wonderful article on the Advanced Aquarist website that summarizes the whole new thinking about the nitrogen cycle, The Nitrogen Cycle, New Developments and New Prospects by Marco Colasanti. 

29 gallon, March 29th, 2016. 
Notes to pictures. The Wisteria in a pot was an experiment, using a light layer of my substrate, then some organic potting soil, and finally a layer of gravel from my 5 gallon tank, to get a bacterial culture started. The plant also came from my 5 gallon tank. The Wisteria is taking over the tank, and I've planted 5 shoots around the pot. I've added some Java Fern from the old tank on the drift wood, and since the beginning I've had some Java Moss with the drift wood also. The two Amazon Sword plants were store bought, and they sprouted 3 shoots, which I have planted as their roots grew large enough. The Marimos came from the old tank as well, and in the foreground are some micro swords that are also transplants. 

What we used to know

Graph of the nitrogen cycle in a new tank
http://answers.seneye.com/@api/deki/files/61/=seneye_new_tank-02.png

What we used to know about the nitrogen cycle, is what it looks like on the surface, as in the above grapha, which seemed to be fairly linear, was this:
  1. The first stage was the conversion of ammonia to nitrite, 
  2. then nitrite to nitrate, 
  3. and finally the nitrate can only be handled to a degree by plants (because it is a nutrient to them) and for the rest by water changes.
But what we used to know is not all there is to know for there are more processes involved in the mature nitrogen cycle than the seemingly linear progression suggested by the observation of how it evolves in a new tank.

What we know now

New research that has been summarized by Colasanti shows that there are three other processes going on, and the whole thing is made up of interlocking cycles, and not linear at all (here are the three new processes from the article):
  1. Ammonium oxidation by a particular group of microorganisms, the archaeabacteria (AOA)
  2. The anaerobic reduction of nitrates to ammonium (DNRA):
  3. The anaerobic oxidation processes of ammonium (ANAMMOX)
In short, the nitrogen cycle is far more intricate than we knew, and now the interesting issue is, can we learn to manage it better, especially during startup of a new aquarium. And the answer is we can. For now, I will write up how I would like to manage the setup of my next tank, based on what I have learned from this one. For that it is important to keep your eyes on the Relative Hydrogen Score referred to above.
The complete Nitrogen cycle - from: http://www.advancedaquarist.com/2011/4/chemistry


My Ideal Aquarium Startup

Based on what I have gradually learned from starting up my current 29 gallon tank, my ideal start up would be much more deliberate, and I would keep a record of daily results for the first two months, including Ammonia, Nitrite, Nitrate, pH, ORP, KH, GH, and TDS. For my pH and TDS toolset I would use electronic probes, and for my Ammonia I would rely first and foremost on the Seachem Ammonia Alert mentioned above, because it measures only the free ammonia (NH3), and not the ammonium (NH4+). Traditional tests like the API ammonia test really give you the TAN (Total Ammonia Nitrogen) content of the water, i.e. both NH3 and NH4.
Constant monitoring is crucial during the early going especially. Once your tank is up and running you will still want to do monitoring, but you can do it more on an exception basis, and once a week, or even less, suffices.

Tools for Aquarium Monitoring

With the above, you have the ability to do daily testing with ease, and with high accuracy. Currently I am working without an accurate pH probe, so all I have is the API test kit which has a range of 6.0-8.0, and it leaves me hanging for readings outside of that, which limits the accuracy of my rH computations.

Other startup supplies

  • Seachem Prime, water conditioner and useful to limit problems; even if you do have an ammonia spike, like when some creature died (you can't even always find them...). or you can use Seachem Amquel when it's just about an ammonia spike.
  • Seachem Stability, an effective way to support bacterial cultures to minimize New Tank Syndrome (NTS)
  • Seachem Replenish, mineral supplement, to provide the proper electrolytes to the water - essential if you use RO/DI water, or even if you use a ZeroWater filter, like I do.
  • for regulating the pH, you can use the Acid/Alkaline regulators, which provide the water with buffering capacity, and are probably a better bet than the neutral regulator, which sometimes is not without side effects, because it works with phosphates.
  • Note, if you are using tap water, you need to be using regular water conditioners, such as Prime and Stability. Obviously, for specific fish you may need special conditioners, and Salt Water is a whole separate discussion.
  • To start the bacterial culture in my substrate, I also used, and will use again in future, the Prodibio BacterKit Soil, which I applied in my current tank in about week three, but ideally you should use it when you set up the tank.

The Startup Process for a Planted Tank

I am being specific for a planted tank. I don't know any other. I've never really run a tank without plants, and the longer I go, the more important the plants are to me.
  1. Preparing the tank as you always would, rinsing it out first.
  2. Install under-gravel heating cable. Highly recommended because it provides circulation in the gravel.
  3. Install the appropriate substrate, and treat with the Prodibio BacterKit Soil.
  4. Fill up slowly with water.
  5. Adjust the water parameters (pH, KH, GH), with Acid/Alkaline buffers, and/or Replenish, and if need be some baking soda to get your KH to where it needs to be (50-100 for the plants, and for healthy nitrifying bacteria).
  6. Get filtration going, and ideally introduce plants, filter foam, and/or gravel from a healthy tank. Ideally do a full weak treatment with Stability, to seed the filter with healthy bacteria.
  7. Add some Wondershell from AAP - the regular kind, not the medicated kind. Wondershell is a calcium/magnesium block which seems to help improve the ORP (negative shift), and get the rH lower, indicating healthy water conditions. It does not significantly affect the pH but it causes a slight bump in GH, depending on how much you use.
  8. Introduce plants, remember to include some from established aquariums (to transfer good bacteria).
  9. My first suggestion would be to start with an invert culture, at a minimum some Malaysian Trumpet Snails, but possibly more.
  10. Gradually introduce some fish.
  11. Monitor all your parameters like a hawk for the first two months.
  12. If need be, manage all water parameters, but especially keep pH within reason, plus or minus 0.2 from where you want to be, and if you have an ammonia spike, remember it is less toxic below a pH of 6.4, but about 50/50 at the neutral range of 7.0. You want to keep your Prime, or Amquel and Stability at the ready.

Here are some initial observations:

  • My ZeroWater filtered water has a pH of 6 or lower (TDS 000-006), and an ORP of about 220, and therefore the rH is nearly neutral at about 26, which is just slightly below the neutral point of 28.
  • In my ATO tank I have a small AAP wondershell, and the parameters change to pH 6, TDS 36, and ORP 125, for an rH of 23, meaning that this water now is significantly reducing.
  • In my tank my TDS is nearly 500, pH 6.8-7.0, and the ORP is ca 80 (it seems to be ca 10 points higher at night and 10 points lower by day). In short the rH now is 23.43, again very solidly reducing. 
The above corresponds to the findings on the AAP website on the healthy range of ORP in a tank, but I suspect that in the long run it will be very rewarding to focus on the rH directly. It does seem that it is the biological (bacterial) activity in the tank that causes the further left shift in the ORP, and would enable lower rH, and thus more Hydride (H- ions, negative Hydrogen ions, or Hydride) in the water, giving it anti-oxidant properties.
quote
Bacterial Activity
Bacterial action, by both anaerobic and aerobic bacteria, can significantly change the ORP of a mass of water (underground aquifer, lake, stream, bay) over time, due to biochemical action. Generally, this shift is in the negative direction, toward a lower ORP, but the direction and amount of shift in ORP will depend entirely upon the bacteria present, bacterial foods, other substances present in the water, as well as temperature and other factors.
unquote (from: h-minus-ion.vpinf.com/Methods-to-modify-ORP-water-1.html)
I use a spreadsheet for my water parameters, and it computes the rH automatically. For the moment, my limitation is my API pH water test, but once I switch to an electronic tester, my pH readings will become even more accurate.

Friday, March 4, 2016

On Water Quality

It's one thing if you keep an aquarium, or aquariums in a house you own, you have no problem making the permanent plumbing fixes that you need to support your hobby, but it gets different in a rental apartment.

To make your own water or not 

For a salt water aquarium, you will have to, but for freshwater maybe you don't, unless you are a stickler like me. How could I not? For myself, I don't use tap water, so why would I for the fish? Personally, I use the ZeroWater filter, which does a pretty thorough job. Then I use Concentrace mineral supplement to add back the good electrolytes.
For the aquarium I use the same ZeroWater, with Seachem Replenish, to replace the necessary minerals (adjusting GH), and I may use some sodium bi-carbonate (baking soda) to adjust KH, as well as the Seachem alkaline and acid buffers, if needed. After a while, you get handy at keeping the water parameters in check.
Now that my 29 gallon tank is operational, I have plain ZeroWater in a top-off reservoir, and when I do a water change I will supply the necessary minerals, but for top off water I need only the pure zero water, since in that case evaporation leaves the minerals behind.

Since my discovery of the wealth of information on the AAP website, I began to explore using the Wonder Shell-Aquarium Mineral Block that they so highly recommend - obviously, I use the plain, non-medicated ones. Presently, I don't have long enough experience with this product to tell you if I like it or not, but the explanation made sense, so I have added it to my routine, and we'll see how well we do...



The first picture shows the basic setup. Very sparse for now, and all I have are some inverts, Malaysian Trumpet Snails, tubifex et al., seen here devouring a blanched leaf of organic spinach. There are also a half dozen of Amano shrimp around.

Starting up the tank

This time I decided to build up the tank very slowly, and I will stick with the invert cultures for a while, before I add any fish. I think I may have a little plantation of Amazon swords on my hands... So far I have not yet attempted water changes on the new tank, but I'm monitoring water conditions on a weekly basis. The wisteria in a terracotta pot is an experiment, for in the pot is some garden soil, covered with some gravel from the previous aquarium, thus providing a starter culture for healthy bacteria growth, and combining that with my very slow path of adding occupants to the tank, there will never be an opportunity for the dreaded "new tank syndrome." So far Ammonia and Nitrates consistently read zero, and nitrates and phosphates are not an issue, vindicating this slow buildup of the water conditions.
The AAP article on aquarium cycling is here, and is one of the best resources you can find. 

The only plants I have right now are easy ones that do not need a lot of light, and I use some Seachem Excel as well as a few Flourish tabs to help things along. In all, I am trying to keep PH at 6.8-7.0, and KH and GH between 100-200 ppm. Clearly, the Amazon swords are doing really well. Soon I will have a little plantation going on. All of which is fine by me, at this stage of the tank all I want is a few plants, just to grow a natural environment, and then in a while I plan to do a proper aquascaping project, once I have CO2 and serious lighting. The most interesting thing that I will want to observe is to what extent the plants and the filter compete or complement each other. Diana Walstead does her aquariums without filtration because the plants are the filter, and with heavier planting that would be true, nevertheless in my little 5 gallon nano tank, I still tend to see some nitrates and phosphate, and now I am trying to grow some duck weed, to see if that mitigates it. I also have reduced the population a bit, since moving the Amano shrimp to the 29 Gallon tank. Clearly, establishing a healthy plant life is the next most important thing after getting the nitrogen cycle started. In the beginning I'm using some fertilizer, and with increasing biological activity in the aquarium, I should be able to reduce my reliance on fertilizer - that will be a focus of my experimentation in the future.

What is most interesting about the information from the American Aquarium Products website, is the focus on ORP, oxidation reduction potential, This makes a lot of intuitive sense, since it would indicate the health of the water, and support a healthy immune system in your fish. For human beings it is known that many healing waters have extreme negative ORP, which is the reason for the popularity in certain quarters of water electrolyzers, popularly known as ionizers, to produce Electrolyzed Reduced Water (ERW). Clearly, the nitrogen cycle would cause a shift towards lower ORP, and thus healthier water. At some point I am planning to look into this more deeply.